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Datasheet
Monoclonal Antibody to Basophil (Clone 212H6.16) Alexa 647 Conjugated
Cat.No DDX0030A647
Description Basophil
Format Alexa Fluor® 647
Unit 0.1 mg
Price(USD) 455.00
MSDS N/A
Catalog No
:
DDX0030A647
Contents
:
100 µg in 200µl PBS 50% glycerol
Isotype
:
IgM
Clone
:
212H6.16
Purification
:
Purified using a QMA Hyper D ion exchange column
Species
:
Human
Host
:
Mouse
Supplier
:
Dendritics
Market Availability
:
North America (United States & Canada)
Application
Flow (Cell Surface): 5-20ug/ml* IF/ICC: 5-20ug/ml* Storage Aliquot and store at -20°C, protected from light. Contains no preservative; KEEP STERILE; AVOID REPEATED FREEZE/THAW CYCLES.
Background Basophils and mast cells are multifunctional hematopoietic effector cells involved in allergic and inflammatory reactions. Immediate allergy is caused by release of chemical mediators by these cell types. In human peripheral blood, 212H6.16 is an antibody specific to a yet non-identified cell-surface structure present on basophils. 212H6 does not induce basophil degranulation, and is therefore a valuable tool for the isolation and study of human basophils. Tonsillar epithelium and skin keratinocytes are also stained by 212H6 in situ: antigen expression in tonsil is restricted to cells lining the epithelial crypts.
Antigen
Enriched-human tonsillar pDCs
Application Notes
*Optimal dilution should be determined by each laboratory for each application
FACS staining: A488-212H6 expression on human PBMC.
Giemsa staining of fresh MACS –separated 212H6+ cells. Basophils are un-degranulated.
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Reference 1. Bendriss-Vermare N.et al., Leukocyte Typing VII, White Cell Differenciation Antigen MC13, part 9, myeloid cells, page 425-30, year 2001, Editor Mason.
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase. Alexa Fluor® is a registered trademark of Molecular Probes, Inc. IMGENEX sells Alexa Fluor® dye conjugated antibodies under license from Molecular Probes, Inc. for research use only.
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DDX0030A647 [Monoclonal Antibody to Basophil (Clone 212H6.16) Alexa 647 Conjugated]
