Newsletter Signup
     
Product
 
   
uyyhu
Resources & Support
  Technical Support
  Protocols  
  Knowledgebase/FAQ  
  Technical Articles  
  Downloads  
  RNAi Resources  
  Product Citations  
Please contact your local IMGENEX distributor for pricing on these products. For a list of distributors, click here or go to www.imgenex.com/distributors.php

Datasheet

 
Monoclonal Antibody to GAPDH - Loading Control
Cat.No
IMG-5019A-2
Description
GAPDH - Loading Control
Format
T.C. Sup.
Unit
0.1 mL
Price(USD)
270.00
MSDS
N/A
Catalog No : IMG-5019A-2
Contents : 100 ul antibody solution containing 50% glycerol, 0.01% thimerosal and 1.0 mg/ml BSA.
Isotype : Mouse-IgG1
Clone : GAPDH 1D4
Species : Bovine, Chicken, Human, Mouse, Pig, Rabbit, Rat
Host : Mouse
Application
IF/ICC: 1:100-1:1000
IP: see Kang et al
Western blot analysis: 1:1000-1:5000
Storage
Store at -20 degrees C. Avoid multiple freeze/thaw cycles.
Recommended Positive Control : HeLa, Jurkat, Daudi, 293, Rh30, A375m T98Gm HCT-116, Hep-2, ovary, stomach, lung, liver, heart.  Most cell lines and tissues will be positive for GAPDH. 

Background
Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH, G3PDH or GPDH) is one of the key enzymes involved in glycolysis; it catalyzes the reversible oxidative phosphorylation of glyceraldehydes-3-phosphate. The GAPDH gene is constitutively and stably expressed at high levels in almost all tissues and cells, and as such is considered to be a “housekeeping” gene. Housekeeping proteins like GAPDH are useful as loading controls for western blots and protein normalization. They are also useful for visualizing cells in microscopy assays.

Antigen
Purified pig GAPDH enzyme.

Application Notes
On Western blots, GAPDH is detected as a band of approximately 36-40 kDa. GAPDH antibodies are widely used as loading controls for quantitative Western blotting, including normalizing Western blot results to GAPDH. Species reactivity: GAPDH is highly conserved across species, and antibodies to GAPDH typically have broad species reactivity. Clone 1D4 has been shown to react with human, cow, pig, mouse, rat, and chicken GAPDH. Since GAPDH is highly conserved across species, it is likely that the antibody reacts with additional species.

Genebank Info (Protein)
NP_002037

 

Application of the GAPDH antibody as a protein loading control in Western blots. Total proteins from various human cell lysates were normalized using the GAPDH monoclonal antibody. The blot shown is IMGENEX's INSTA-Blot: Human Cell Lines, Cat. no. IMB-105.

Application of the GAPDH antibody to visualize cells by fluorescence microscopy. Sh-SY5Y human neuroblastoma cells were stained with GAPDH, followed by an FITC labeled second step antibody (green) and counterstained with Hoechst dye (blue).

Application of the GAPDH antibody as a protein loading control in Western blots. Total proteins from various rat tissue lysates were normalized using the GAPDH monoclonal antibody. Rat tissue lysates, Cat nos: Ovary (40151), Stomach (40130), Lung (40126), Liver (40125), and Heart (40122).  

ffsdf

Related Products

1. 20101 [Goat Anti-Mouse Ig HRP Conjugate ]
2. 40161 [HeLa cell line lysate (cervical carcinoma)]
3. 40163 [Daudi cell line lysate (lymphoma, Burkitt)]
4. 40164 [HEK293 cell line lysate (embryonic kidney)]
5. 40166 [Rh30 cell line lysate (rhabdomyosarcoma)]
6. 40151 [Human Ovary Tissue lysate]
7. 40152 [Human Stomach Tissue lysate]
8. 40146 [Human Lung Tissue lysate]
9. 40145 [Human Liver Tissue lysate]
10. 40142 [Human Heart Tissue lysate]
11. IMG-6166K [Western Blot Loading Control Sampler Kit]

Reference


Product Citations
1. Increased susceptibility to transcriptional changes with novel stressor in adrenal medulla of rats exposed to prolonged cold stress. Xiaoping Liua, Richard Kvetnanskyb, Lidia Serovaa, Anne Sollasa and Esther L. Sabbana. Molecular Brain Research, doi:10.1016/j.molbrainres.2005.07.019 On line publication. 
2. Role of mouse Hepatitis Virus (MHV) receptor murine CEACAM1 in the resistance of mice to MHV infection: Studies of mice with chimeric mCEACAM1a and mCEACAM1b. Hirai A, N Ohtsuka, T Ikeda, R Taniguchi, D Blau, K Nakagaki, H Miura, Y Ami, Y Yamada, S Ito, K Holmes, F Taguchi. J Viro 84: 6654-6666 (2010).
WB: The liver, spleen, and ileum of MHV-A59 infected mice, Fig 3A.
3. Dimethyl Sulfoxide Has an Impact on Epigenetic Profile in Mouse Embryoid Body. Misa Iwatani, Kohta Ikegami, Yuliya Kremenska, Naka Hattori, Satoshi Tanaka, Shintaro Yagi, and Kunio Shiota. Stem Cells, 24: 2549-2556 (2006).  
4. Allelic Variation in the Hepatitis C Virus NS4B Protein Dramatically Influences RNA Replication. K J. Blight, J. Virology., DOI:10.1128/JVI.02481-06 (2007). Imgenex antibodies cited: 1. GAPDH (IMG-5019A-1, IMG-5019A-2) [WB, Fig7, Fig8 (Huh-7.5 human hepatoma cell line)].
5. Membrane localization and pH-dependent transport of a newly cloned organic cation transporter (PMAT) in kidney cells. L Xia, K Engel M Zhou, and J Wang. Am J Physiol Renal Physiol 292:682-690 (2007). Imgenex Products cited: 1. INSTA-Blot™ Human Tissue: ready-to-use WB (IMB-103); 2. GAPDH (IMG-5019A-1/IMG-5019A-2). [WB: Fig 2, human kidney tissue]

6. Cdc42-interacting protein-4 functionally links actin and microtubule networks at the cytolytic NK cell immunological synapse. Banerjee P., R. Pandey, R. Zheng, M. Suhoski, L. Monaco-Shawver, J. Orange. J. Exp. Med. 10.1084/jem.20061893 (2007). WB (Fig 8A): Protein standardization for siRNA studies
7. Cadiomyocyte apoptosis in autoimmune cardiomyopathy: mediated via endoplasmic reticulum stress and eggerated by norepinephrine. Mao W, S fukuoka, C Iwai, J Liu, VK Sharma, S-S Sheu, M Fu, and C-s Liang. Am J Heart Circ Physiol 293:H1636-H1645 (2007). Imgenex antibodies cited:
1. ATF6 (IMG-273): WB (Fig 7), analysis of ATF6 in nuclear fraction of rabbit left ventricle myocardium tissue lysates,  Authors describe cleaved forms of p90ATF6 in the nucleus: p60ATF6 and p36ATF6.
2. GAPDH (IMG-5019A): WB (Fig 4), GAPDH antibody used as a loading control in rabbit left ventricle myocardium tissue lysates.
    
8. Metformin transport by a newly cloned proton-stimulated organic transporter (Plasma membrane monoamine transporter) expressed in human intestine.  Zhou M, L. Xia, J Wang Drug Metabolism and Disposition DOI:10.1124/dmd.107.01549 (2007).  Imgenex products cited for WB (human small intestine, Fig 6B): 
1. GAPDH (IMG-5019A-1, -2)
2. INSTA-BLOT containing human small intenstine tissue lysate (IMB-103, IMB-123)

9. Estrogen effects on Skeletal muscle inslulin-like growth factor-1 and myostatin in ovariectomized rats.  Zhou M, L Xia, J Wang. Experimental Biology and Medicine 232:1314-1325 (2007). WB (used as a gel loading control for rat muscle tissue lysates): Figs 6C, 7C, 8C.
10. Emerging role for autophagy in the removal of aggresomes in Schwann Cells. Fortun J, W Dunn, S Joy, J Li, L Notterpek. J Neurosci 23: 10672-10680 (2003). WB: GAPDH used as a protein loading control (Mouse Schwann cell lysates), Fig. 3F.
11. Cathepsin B mRNA and protein expression following contusion spinal cord injury in rats. Ellis R, J Earnhardt, R Hayes, K Wang, D Anderson. J Nuerochem 88: 689-697 (2004). WB: GAPDH used as a protein loading control (rat spinal cord tissue), Fig. 3a,b,c.
12. The extensive nitration of neurofilament light chain in the hippocampus is associated with the cognitive impairment induced by amyloid Β in mice. Alkam T, A Nitta, H Mizoguchi, A Itoh, R Murai, T Nagai, K Yamada, T Nabeshima. JPET 327: 137-147 (2008). WB (mouse hippocampal lysates), Fig. 7A.
13. RNAi-mediated silencing of nuclear factor erythroid-2-related factor 2 gene expression in non-small cell lung cancer inhibits tumor growth and increases efficacy of chemotherapy. Singh A, S Boldin-Adamsky, R Thimmulappa, S Rath, H Ashush, J Coulter, A Blackford, S Goodman, F Bunz, W Watson, E Gabrielson, E Feinstein, S Biswal. Cancer Research 68: 7975-7984 (2008). WB (human A549 and H460 cells), Fig. 1.
14. Transcriptional restriction of HIV-1 gene expression in undifferentiated primary monocytes.  Dong C, C Kwas, L Wu. Journal of Virology doi:1128/JVI.02665-08 (2009). WB (human monocytes), Figs. 5A,C; 7A.
15. A gogli-associated protein 4.1B variant is required for assimilation of proteins in the membrane.  Kang Q, T Wang, H Zhang, N Mohandas, X An. Journal of Cell Science 122: 1091-1098 (2009). IP (human bronchial epithelial cells), Fig. 7A.
16. Iris pigment epithelial cells express a functional lipopolysaccharide receptor complex. Chui J, M Li, N Girolamo, J Chang, P McCluskey, D Wakefield. Inves Opthalmology & Vis Sci 51:2558-2567 (2010). GAPDH (IMG-5019A-1): WB, human ocular pigment epithelial and normal aqueous cells, Fig 3.  
17. Pannexin 2 is expressed by postnatal hippocampal neural progenitors and modulates neuronal commitment. Swayne L, C Sorbara, S Bennett. J Bio Chem 285: 24977-24986 (2010).
WB: Neural stem and progenitor (NPC) cells isolated from postnatal day 3 C57BL/6 mouse hippocampus, Fig 1A.
WB: Neuronal cells isolated from 14 day old C57BL/6 mouse neurospheres, Fig 2C & 3C.


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.

Related Products

Related Product(s) of the Catalog # IMG-5019A-2
Cat. No Description Format Species Appl. Qty Price(USD)
20101 Anti-Mouse Ig HRP Conjugate HRP M ELISA, WB 0.1 mL 80.00 Add to Cart
Cat. No Description Species Qty Price(USD)
40161 HeLa cell line lysate (cervical carcinoma) H 0.1 mg 84.00 Add to Cart
40163 Daudi cell line lysate (lymphoma, Burkitt) H 0.1 mg 84.00 Add to Cart
40164 HEK293 cell line lysate (embryonic kidney) H 0.1 mg 80.00 Add to Cart
40166 Rh30 cell line lysate (rhabdomyosarcoma) H 0.1 mg 84.00 Add to Cart
40151 Human Ovary Tissue lysate H 0.1 mg 84.00 Add to Cart
40152 Human Stomach Tissue lysate H 0.1 mg 84.00 Add to Cart
40146 Human Lung Tissue lysate H 0.1 mg 84.00 Add to Cart
40145 Human Liver Tissue lysate H 0.1 mg 84.00 Add to Cart
40142 Human Heart Tissue lysate H 0.1 mg 84.00 Add to Cart
Cat. No Description Format Species Appl. Qty Price(USD)
IMG-6166K Western Blot Loading Control Kit Kit M WB 0.05 mg each 420.00 Add to Cart

FAQ

Welcome to the IMGENEX Knowledgebase Center!

The IMGENEX Knowledgebase Center is an interactive tool containing hundreds of answers to frequently asked technical questions compiled by IMGENEX technical support scientists. Updated daily, you can quickly find information about specific products and applications.


Searching the IMGENEX Knowledgebase Center

You can search the Knowledgebase using two different search methods. To quickly search for all tech notes and inquiries related to a specific keyword or application simply enter a keyword into the search box and click the Search button. To browse technical inquiries by product or category, use the links below to navigate to the appropriate product platform.

Search the IMGENEX Knowledgebase by Keyword  


IMG-109 [Polyclonal Antibody to DR4/TRAILR1 (NT)]
Go to 'IMG-109' Data Sheet

Protocols

Imgenex Protocols


No Result Found...!

dfgd
Product Search
dfgd
dfgd
dfgd
dfgd
dfgd
dfgd
dfgd
Privacy Policy | Site Map | RSS | About us | Products | Promotional Products | Resources | Order Form | Tech Services | Newsletter | Custom Services | Distributors | Knowledgebase Center
© Imgenex Corporation, Toll Free: 1-888-723-GENE(4363) Phone: 1-858-642-0978 Fax: 1-858-642-0937