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Datasheet
Polyclonal Antibody to mouse TLR3 (Toll-like receptor 3)/CD283
Cat.No IMG-516
Description TLR3/CD283
Format Purified
Unit 0.1 mg
Price(USD) 255.00
MSDS
Catalog No
:
IMG-516
Contents
:
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype
:
Rabbit IgG
Purification
:
Protein G Chromatography
Species
:
Human, Mouse
Predicted React
:
Rat
Host
:
Rabbit
Application
Flow (Cell Surface):see Pawar et al. (2006) for details Flow (Intracellular): 1 ug/10^6 cells IHC (frozen): see Pwar et al, 2005 for details. IHC (paraffin): 1:50 (see Patol et al, 2005 for details) Western blot analysis: 1-3 ug/ml Storage Store at 4°C, stable for 6 months. For long-term storage, store at -20°C. Recommended Positive Control : Mouse spleen
Background The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). mTLR3 cDNA codes for a protein with approximate molecular weight of 100 kDa . TLR3 has a restricted expression pattern being expressed in dendritic cells (DC) (4-5). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes. The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting.
Western blot analysis of TLR3 in mouse spleen tissue lysate using IMG-516 at 2 ug/ml.
Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes using IMG-516 at 1 ug/10^6 cells. Shaded histogram represents cells without antibody; green represents rabbit IgG isotype control (IMGENEX, 20304); red represents anti-TLR3 antibody. Goat anti-rabbit IgG FITC (IMGENEX, 20302) was used as secondary antibody.
Reference 1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A.
J. Exp. Med. 187: 2097-2101 (1998). 2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997). 3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001) 4. Muzio M, Polentarutti N, Bosisio D, Prahladan MK, Mantovani A. J Leukoc Biol 67(4):450-456 (2000). 5. Muzio M, Bosisio D, Polentarutti N, D'amico G, Stoppacciaro A, Mancinelli R, van't Veer C, Penton-Rol G, Ruco LP, Allavena P, Mantovani A. J Immunol 164(11):5998-6004 (2000).
Product Citations 1. Viral Double-Stranded RNA Aggravates Lupus Nephritis through Toll-Like Receptor 3 on Glomerular Mesangial Cells and Antigen-Presenting Cells. Prashant S. Patole, Hermann-Josef Gröne, Stephan Segerer, Raluca Ciubar, Emilia Belemezova, Anna Henger, Matthias Kretzler, Detlef Schlöndorff, and Hans-Joachim Anders. J. Am. Soc. Nephrol., Mar 2005; 10.1681/ASN.2004100820. Imgenex antibodies cited: 1. TLR3 (IMG-516) [IHC-P, Fig.2 (MRL 1pr/1pr mice kidneys)]. [Flow (cell surface and intracellular), Fig.3 (murine mesangial cell line)]. 2. Toll-Like Receptor-7 Modulates Immune Complex Glomerulonephritis. Pawar R.D., P.S. Patole, D. Zecher, S. Segerer, M. Kretzler, D. Schlondorff, and H-J Anders., J Am Soc Nephrol, 17: 141-149 (2006). Imgenex antibodies cited: 1. TLR3 (IMG-516) 2. TLR7 (IMG-581) [Flow (intracellular and cell surface), Fig.3: TLR3 (mesengial cells and J774 macrophages), TLR7 (J774 macrophages)]. [IHC-F, Fig.2 (kidneys of MRL1pr/1pr mice)]. 3. Expression and Regulation of Toll-like Receptors in Lupus-like Immune Complex Glomerulonephritis of MRL-Fas(lpr) Mice. Patole PS, RD Pawar, M Lech, D Zecher, H Schmidt, S Segerer, A Ellwart, A Henger, M Kretzler, and H-J Anders. Nephrol Dial Transplant, 21:3062-3073 (2006). Imgenex antibodies cited: For IHC (frozen) on mouse kidney sections (Fig. 5): TLR3 (IMG-516), TLR7 (IMG-581A), TLR9 (IMG-431) and Rabbit IgG isotype control antibody (20304). For Flow (Intacellular) and Flow (Cell surface) on J774 mouse macrophage-like cell line (Fig 6B): TLR7 (IMG-581A), TLR9 (IMG-431) and Rabbit IG isotype control antibody (20304). 4. Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth. Cho WG, RJC Albuquerque, ME Kleinman, V Tarallo, A Greco, M Nozaki, MG Green, JZ Baffi, BK Ambati, M De Falco, JS Alexander, A Brunetti, S De Falco, J Ambati. PNAS 106:7137-7142 (2009). IMGENEX products cited.1. IMG-516 (TLR3 pAb): IHC (frozen), Figs 3A,B and S1A, B (mouse corneas and skeletal muscle ) .2. IMG-5348A [TLR3/CD283 (Phospho Tyr 759)]: WB, Fig 4D (mouse primary lymphatic endothelial cell cultures). Note: exposure to 21nt-siRNA-luciferase triggered TLR3 phosphorylation within 5 min after stimulation. 5. Sequence- and target-independent angiogenesis suppression by siRNA via TLR3. Kleinman ME, K Yamada, A Takeda, V Chandrasekaran, M Nozaki, JZ Baffi, RJC Albuquerque, S Yamasaki, M Itaya, Y Pan, B Appukuttan, D Gibbs, Z Yang, K Kariko, BK Ambati, TA Wilgus, LA DiPietro, E Sakurai, K Zhang, JR Smith, EW Taylor, J Ambati. Nature 452:591-597 (2008). IMGENEX products cited: 1. IMG-516 (TLR3 pAb): IHC (frozen), Fig S9a (Mouse eye: TLR3 and CD31 signals colocalized at the site of laser injury) 2. IMG-315D (TLR3-PE mAb): Flow (intracellular) and Flow (cell surface), Figs S9f,g (human choroidal endothelial cells) and Fig 26 (human aortic, dermal microvascular, pulmonary microvascular and umbilical vein endothelial cells). TLR3 was detected intracellularly as well as on the cell surface.
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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