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Datasheet
Peptide-affinity Purified Polyclonal Antibody to Influenza A H1N1 NS
Cat.No IMG-6461A
Description Influenza A H1N1 NS
Format Purified, Peptide Affinity
Unit 0.1 mg
Price(USD) 310.00
MSDS
Catalog No
:
IMG-6461A
Contents
:
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype
:
Rabbit Ig
Purification
:
Antigen Affinity Chromatography
Host
:
Rabbit
Application
Western blot analysis: 0.1-1 ug/ml Storage Store at 4°C, stable for 6 months. For long-term storage, aliquot and store at -20°C.
Background On 6/09/2009, the World Health Organization classified swine flu influenza (H1N1) to be the first influenza pandemic of the 21st century. Elucidating risk factors, illness patterns and transmission characteristics are among the highest priorities in H1N1 epidemiological studies.
Influenza A H1N1 NS, also known as Non-structural protein 1 or NS1A or NS1, is a 234 amino acid containing protein belonging to the Influenza A virus (strain A/Swine/Iowa/15/1930 H1N1) which has aves, human and pig as host. This protein belongs to the influenza A viruses NS1 family and is a homodimer located in the nucleus of uninfected, transfected cells, and in the cytoplasm of infected cells. The viral protein NS1 binds to and inhibits two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs which include the 30 kDa cleavage and polyadenylation specificity factor (CPSF4) and the poly(A)-binding protein 2 (PABPN1). Thus inhibiting post-transcriptional processing of cellular pre-mRNA and leading to accumulation of unprocessed 3' end pre-mRNAs in nucleus and loss of cellular protein synthesis. This helps the virus to escape interferon synthesis after activation of the IRF3 pathway, and therefore prevents establishment of cellular antiviral state. NS1 is also known to prevent human EIF2AK2/PKR activation, at the very beginning of the infection, when NS1 is mainly present in the cytoplasm and prevent antiviral response.
Antigen
Amino acids 205-220 (SRNENERPSLPPKQKR) of human Influenza A H1N1 NS were used as the immunogen.
Western blot analysis of Influenza A H1N1 NS in a partial recombinant fusion protein containing amino acids 205-220 using IMG-6461A at 0.1 ug/ml. IMGENEX’s goat anti-rabbit Ig HRP secondary antibody (IMGENEX, 20301) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test.
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Reference 1. Nakajima K. et al. Virology. 139:194-198 (1984). 2. Krug RM. Virology. 309:181-189 (2003).
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
Related Products
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IMG-6461A [Peptide-affinity Purified Polyclonal Antibody to Influenza A H1N1 NS ]
