Please contact your local IMGENEX distributor for pricing on these products. For a list of distributors, click here or go to www.imgenex.com/distributors.php
ChIP Assays and Components from IMGENEX:QuikChIP
Chromatin Immunoprecipitation Kits & Reagents
Chromatin Immunoprecipitation (ChIP) is a powerful method used to identify regions of the genome associated with specific proteins. These associations are crucial for vital cellular functions including gene transcription, DNA replication and recombination, repair, segregation, chromosomal stability, cell cycle progression, and epigenetic silencing. The ChIP assay has been used to study both histones and non-histone proteins, such as transcription factors, within the context of the cell. Transcription factors and other DNA binding proteins have a weaker affinity than histones, which generally are tightly associated within the chromatin complex. To avoid dissociation of non-histone proteins from the chromatin binding site, it is necessary to incorporate a cross-link step such as formaldehyde to cross-link proteins to DNA. After cross-linking occurs, the chromatin are released from the nuclei and sheared into 200-1000bp fragments. The chromatin are then immunoprecipitated with specific antibodies. DNA sequences cross-linked to the protein of interest co-precipitate as part of the chromatin complex. The DNA/ chromatin/antibody complex is then isolated using Protein G agarose. After reverse cross-linking, the associated DNA is released from the complex and is ready for analysis.
Overview of the ChIP Process
Tools for Effective Chromatin Immunoprecipitation
The QuikChIP™ difference
The principle of the ChIP assay is simple: selective enrichment of a chromatin fraction containing a specific antigen. However, the process can be technically challenging. Depending on the protein of interest and starting material, appropriate antibodies may be difficult to come by. Not only must the antibody be able to bind to the protein in its native state, the epitope it recognizes can't be cloaked by the DNA. Beyond the challenge of finding an acceptable antibody, the outcome of your ChIP experiment also depends on the correct formulation of several different reagents and buffers. It is with these technical challenges in mind that IMGENEX developed QuikChIP™ kits and reagents. These products include ready-to-use kits containing critical buffers and inhibitory formulations, ChIP certified antibodies, DNA purification kits, and pre-sheared DNA from cell lysates. Each IMGENEX QuikChIP™ kit or reagent has been carefully developed and tested with ease, simplicity, and quality results in mind.
For faster, more trouble-free results, try our QuikChIP™ Kits and see the difference for yourself! Right now, get a free ChIPClip chip clip (try saying that three times!) with every qualifying order! The ChIPClip chip clip may not make your assay work better, but with it's handy magnet backing its perfect for keeping important documents nearby.
Benefits
Quick & simple
Optimized ready-to-use buffers & protocol are included
Can be used for both histone and non-histone proteins
Reagents for 25 ChIP assays and sheared DNA preparations
