ChIP Assays and Components [QuikChIP™ Certified Antibodies]

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ChIP Assays and Components from IMGENEX: QuikChIP™ Certified Antibodies

The chromatin immunoprecipitation (ChIP) method is a powerful technique to study protein-DNA complexes. The ChIP method allows detection of the interactions between proteins of interest and DNAs with known sequences in vivo. However ChIP requires primary antibodies suitable for immunoprecipitation. These antibodies have an additional requirement in that the epitope recognized by the antibody must be available after binding to DNA. While antibodies useful in immunoprecipitation often work for ChIP, this is not always the case.

A growing number of IMGENEX antibodies have been used in ChIP assays and we are continually testing and validating new products for use in ChIP. Below is a list of our currently available QuikChIP™ Certified Antibodies. Be sure to check back often as we are releasing new antibodies every week!

ChIP Assay Results Using IMGENEX QuikChIP™ Certified Antibodies

Figure 1. ChIP analysis. (A) Telomeric DNA was precipitated by antibodies against the telomeric binding protein TRF2 (IMG-148) but not by non-specific goat IgG. mmunoprecipitation requires formaldehyde cross-linking of proteins to DNA. p53 antibody was not from IMGENEX. (B) Quantitation of the data shown in panel A. was calculated as follows: Percent input precipitated (pptd) = [signal strength of telomeric DNA precipitated with the indicated specific antibody-background signal obtained with non-specific IgG)/input signal] x 100.
(Data courtesy of Dr. Dominique Broccoli. Razak et al. Mol Cell Biol. 24 (13): 5967-5977 (2004).

Figure 2. ChIP-PCR assay for repressive chromatin modifications on the PR promoter CpG island region at 36 and 168 hrs after ERá siRNA treatment. Antibodies against Dnmt1 and Dnmt3b were used in ChIP-PCR assay. After 168 hr of ERá siRNA treatment of MCF-7 cells, recruitment of Dnmt1 and Dnmt3b to the PR promoter was evident. (In: total input, (+) ERá siRNA, (-) without ERá siRNA.
(Data courtesy of Leu et al. Cancer Research 64: 8184-8192 (2004).

Current List of IMGENEX QuikChIP™ Certified Antibodies

Specificity	Host	Reactivity	Applications
Dnmt1	Mouse	M, H	WB, IP, ChIP, IHC (paraffin)
Dnmt3a	Mouse	M, H	WB, IHC, ChIP, IF/ICC
Dnmt3b	Mouse	M, H	WB, IP, ChIP, ICC
HDAC-3	Rabbit	H	WB, IP, ChIP
HDAC-4	Rabbit	M, H	WB, IP, ChIP
HDAC-5	Rabbit	M, H	WB, IP, ChIP
HDAC-6	Rabbit	M, H	WB, IP, ChIP
MBD2	Rabbit	M, H	WB, FA (Neut), ChIP
p53	Rabbit	M, H, R	WB, IP, ChIP, Gel Shift
p73 (Delta-Np73)	Mouse	M, H	WB, IHC (paraffin), ChIP
TRF2	Goat	M, H	WB, IP, ChIP

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