IkBa dominant mutant (IkBaM) is a mutated form of IkBa with a serine-to alanine mutations at residues 32 and 36 (8). Overexpression of IkBaM presumably prevents phosphorylation of native IkBa and there by inhibiting translocation of NF-kB complex to the nucleus and thereby preventing NF-kB activation. In most cell lines, the inhibition of NF-kB activation affects cell growth. The IkBaM was tested on proliferation of HeLa cells in tissue culture plates.