Imgenex siRNA
retriever program has been designed to select siRNA encoding DNA oligonucleotides that can
be cloned into one of the pSuppressor vectors. The input sequence can be directly accessed
from a Genbank accession or sequence provided by the researcher. |
|
| Features |
| 1. |
It will find the coding sequence from the input Genbank
sequence. |
| 2. |
It will add the overhang cloning sequences. |
| 3 |
A choice of loop sequences can be added. |
| 4. |
Allows choosing G/C contents, multiple repeats. |
| 5. |
Length of insert sequence can be custom designed. |
| 6. |
Links to NCBI BLAST program. |
|
| Instructions |
| 1. |
Either enter a Genbank accession number or paste the sequence
into appropriate boxes. |
| 2. |
Choose length of the target sequence in the gene. |
| 3. |
Choose G/C contents, repeat nucleotides. |
| 4. |
View results. |
| 5. |
After viewing the oligonucleotides, do a BLAST search to
identify unique sequence. |
| 6. |
Select loop sequence. The program allows adding predetermined
sequences or custom sequence. Adding a restriction site allows easy identification of
recombinant clones. |
| 7. |
Select the appropriate vector. |
|
