View ALL Related Regulatory T Cell Products 
FOXP3D2 (exon 2 deleted) specific datasheet

FOXP3 is a master regulator of immune homeostasis expressed specifically in CD4⁺ CD25⁺ T regulatory cells controlling their growth, development and function and generally accepted to be expressed in both mouse and human CD4⁺ CD25⁺ regulatory T cells. Whereas mouse is detected as a single band in WB studies, human FOXP3 is consistently seen as a doublet.  The lower band has been identified as a splice isoform lacking exon 2 with a predicted molecular weight of ~4 kDa less than FOXP3.
    
Transient transfection assays using Jurkat cells suggest that the FOXP3D2 is a novel splice variant that functions as a transcriptional repressor protein and acts in cohort with FOXP3 causing a significant suppression of cytokines and up-regulating the expression of various Treg-associated markers.  The existence of functional splice variant forms of FOXP3 suggest an additional level of complexity related to it's biology, although the significance of FOXP3 splice variant forms remains to be elucidated.

As part of a continuing effort to make innovative reagents available to researchers, IMGENEX is excited to introduce a novel FOXP3D2 splice variant specific antibody. Clone 16J4G6 (IMG-6018) specifically detects FOXP3D2 in contrast to other commercially available antibodies which detect both  full-length FOXP3 and FOXP3D2.

Click or call IMGENEX (800-848-9513)
to learn more about this new product!

FOXP3D2 (exon 2 deleted) specific datasheet

View ALL Related Regulatory T Cell Products

The area indicated in orange (Figure A) is coded by exon 2 and is specifically deleted in the FOXP3D2 splice variant. The dark purple coded region (A & B) is the antigenic determinant used for antibody development. IMG-6018 specifically recognizes the new epitope created by the deletion of exon 2.