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Despite intense interest and scrutiny focused on FOXP3 as a key protein & master transcription factor, isolating and enriching for viable FOXP3 positive cells remains a challenge. Although cell separation/staining via CD4+CD25+ selection is commonly used, this technique has limited applications. Thus, surface markers specific for FOXP3 positive cells would be invaluable research tools as they would:
• Facilitate isolation & purification of viable
Treg cells
• Distinguish naturally occurring
CD4+CD25+ cells from both naive and
recently activated CD4+CD25-
nonregulatory T cells
• Allow therapeutic manipulation of Treg cells
As the search for putative FOXP3+ markers continue, Neuropilin-1, GPR83, & FR4 have emerged as potential candidates. IMGENEX is excited to offer a panel of flow cytometric characterized antibodies against:
• Neuropilin-1 (clone 211H6.01)
• GPR83 (polyclonal)
• Folate Receptor 4 (clones 12A5 & TH6) |
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Flow cytometric analysis of intracellular FOXP3 (IMG-5802D) and cell surface FR4 with clone 12A5 (IMG-6217C) (left) and clone TH6 (IMG-6218C) (right) at 0.06 ug/10^6 mouse splenocytes. | |
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Flow cytometric analysis of Neuropilin-1 in CD4+CD25+ human PBMCs using A) an isotype control & B) DDX0440 at 0.5 ug/10^6 cells. | | |
